人白细胞介素-33蛋白的二级结构及B细胞抗原表位预测

Prediction of Secondary Structures and B Cell Epitopes of Human Interleukin-33

目的：预测人白细胞介素（IL）-33的二级结构和B细胞抗原表位。方法：以单参数（亲水性、可及性、柔韧性及抗原性）预测为基础，通过二级结构预测初步筛选，并以ABCpred方案作为最终验证，预测人IL-33蛋白的二级结构和B细胞表位。结果：人IL-33蛋白的N端1—19、59-75、108～117和204～210区段为预测的B细胞表位。结论：该预测结果将有助于确定人IL-33的B细胞表位及其生物学活性部位。

Objective： To predict the secondary structures and B cell epitopes of human interleukin（IL）-33. Methods： The location of B cell epitopes was predicted by screening the secondary structure of IL-33. Analysis was on the basis of the prediction results of hydrophilicity, accessibility, flexibility, antigenicity followed by a verification using ABCpred program. Results： The predicted B cell epitopes were found to locate at the protein sequence of amino acids 1-19, 59- 75, 108-117 and 204-210 for IL-33. Conclusion： These findings would be helpful for estimate of the epitopes and activity sites localized within human IL-33.