人乳头瘤病毒检测在宫颈疾病诊断中的意义

Clinic significance of human papillomavirus detection in diagnosis of precancerous cervical diseases

目的探讨人乳头瘤病毒(human papillomavirus,HPV)联合宫颈液基细胞学(thin-prep cytol-ogy test,TCT)检测在宫颈病变诊断中的临床意义,以及HPV负荷量与宫颈病变程度的相关性。方法对我院妇科2350名患者行宫颈TCT和高危型HPV检测。结果HPV高危阳性患者共895例,阳性检出率为38.09%,TCT结果正常组内:正常、轻度炎症、中度炎症、重度炎症HPV阳性率分别为17.06%、28.30%、36.43%和30.30%;②TCT结果为正常组、ASCUS、ASC-H、LSIL、HSIL中,HPV阳性率分别为27.60%、52.30%、61.31%、86.11%、91.25%。③将HPV负荷量由低至高行数量分级后,TCT异常率分别为12.92%、25.93%、41.38%、50.52%和65.74%。结论在TCT异常者中,病变程度越高,高危型HPV感染率越高。随着病毒负荷量增加,宫颈病变发生,率增加,其病变程度也越重。

Objective To investigate the clinic significance of ThinPrep Cytology Test （TCT） combined with humanpapilloma virus （HPV） DNA hybrid capture Ⅱ （HC Ⅱ） in diagnosis of cervical diseases. To explore the relationship between viral load of HPV and the grade of cervical lesions. Methods Cervical cytological examination and HPV screening were carried by TCT and HC Ⅱin 2 350 women. Results High risk HPV DNA was detected in 895 cases （38.09%）. The HPV infection was 17.06%, 28.30%, 36.43% and 30. 3% in women with normal cytology, mild inflammation, moderate inflammation and severe inflammation, respectively. The HPV infection was 27. 60%, 52.30%, 61.31%, 86.11% and 91.25% in women with normal cytology, ASCUS. ASCH. LSIL, HSIL, respectively. According to the grouping of HPV viral load from low to high, the incidence of abnormal TCT were 12.92%、 25.93%、 41.38%、 50.52%、 and 65.74%、 in turn. Conclusions In the women with abnormal TCT result, the more severe of cervical diseases, the more incidence of high risk HPV infection can be detected. HPV viral load is associated with the incidence and the severity of cervical disease.