局部运用神经生长因子促进骨折愈合的实验研究

Expermental Study on Facilitating Fracture Healing by The Local Application of Nerve Growth Factor

目的:研究神经生长因子对骨折愈合促进的作用,为临床上促进骨折愈合,减少骨不连发生提供有效、安全的治疗方法。方法:在大白兔右侧腓骨中下段截骨制造骨折模型,实验组采用神经生长因子进行治疗,对照组相同剂量的生理盐水进行治疗,在第2、4、6周抽取该组实验兔耳缘静脉血作肾上腺素和去甲肾上腺素测定。在第3、6周后除死大白兔,作病理形态学检查,最后进行统计学处理。结果:骨折后第2、4、6周,在血清中的肾上腺素和去甲肾上腺素浓度实验组高于对照组,差异具有统计学意义(P<0.01或P<0.05)。病理检测实验组在术后第2周纤维骨痂中已有大量透明软骨细胞形成,第6周骨痂中的透明软骨细胞已被成骨细胞替代;而在对照组术后第2周纤维骨痂中仅有纤维组织,而且其间还有破骨细胞存在,术后第4周骨痂中出现了透明软骨细胞,第6周骨痂中的软骨细胞仍没有完全成骨细胞化。结论:神经生长因子对骨折愈合有明显促进作用。

Objectives ： To examine the practical effects of nerve growth factor（NGF）on the fracture healing in vivo and to provide the efficient and safe method of treatment on facilitating facture healing and reducing non--union in clinic. Methods：The rabbit models of the fracture healing were established by recection of 1 cm of the right fibula. Animal models were established in 20 rabbits. 20 model rabbits were divided into 2 groups, with one group （experiment group） receiving NGF, and the other group （control group） receiving the sham treatment as contral. In experiment group, the rabbits serum concentrations of norepinephrine and epinephrine were mensurated respectively after 2,4,6 weeks of operation. 20 rabbits were sacrificed after 3,6 weeks respectively. Specimens of right fibula were sectioned to be studied by histomorphometry study. Results： After 2,4,6 weeks of operation, the rabbits serum concentrations of norepinephrlne and epinephrine were higher than that of control group （P〈0.01 or P〈 0.05）. In experiment group, hyaline cartilage were found in fibrous callus at week 2 post-fracture and mature callus were en- gendered at week 6 post-fracture. In sham group,the fibrous tissue were found in fracture healing site and osteoclasts were also found after 2 weeks. Hyaline cartilage were formed and woven bone were produced after 4 weeks. Conclusion： The local application of NGF could facilitate the fracture healing potently in rabbit.