温度、光照、盐度及pH对旋链角毛藻生长的影响

Effects of water temperature, illumination, salinity and pH on the growth of Chaetoceros curvisetus

以旋链角毛藻(Chaetoceros curvisetus)为实验材料,采用f/2培养基,设置了2个盐度梯度(25和30),4个温度梯度(15℃、20℃、25℃和30℃),3个光照梯度(29.3μE.m-2.s-1、78.12μE.m-2.s-1和126.95μE.m-2.s-1),3个pH梯度(7.0、7.5和8.3)的处理,并计算了不同培养条件下藻细胞在指数生长期的比生长率,比较研究了温度、光照、盐度及pH等环境因子对旋链角毛藻生长的影响。进一步进行盐度、光照的两因素正交实验,并检验两者的交互作用。其中盐度设25、30两个水平,光照强度设29.3μE.m-2.s-1、78.12μE.m-2.s-1和126.95μE.m-2.s-1三个水平。实验结果表明,温度为20℃,光照为78.12μE.m-2.s-1,盐度为25,pH在8.3时是旋链角毛藻的最佳生长条件,此时最大比生长率和生物量达到最高,温度对藻类生长的影响比盐度,光照,pH的要明显得多,实验中其他处理均会抑制其生长。

Chaetoceros curvisetus, which is a red-tide alga, was cultured with f/2 culture medium, and studied at two salinity levels （25 and 30）, four temperature levels （15℃, 20℃, 25℃ and 30℃）, three light intensity levels （29.3 μE·m^-2·s^-1, 78.12 μE·m^2-s^-1 and 126.95 μE·m^-2·^-1 and three pH levels （7.0, 7.5 and 8.3）. The specific growth rates of Chaetoceros curvisetus in exponential growth phase under difterrm culture conditions were also calculated, to address the effects of environmental factors, such as temperature, light intensity, salinity and pH on the growth of Chaetoceros curvisetus.. Moreover, an interaction test between the light intensity and the salinity was studied at three light intensity levels （29.3μE·m^-2s·-^1, 78.12 μE·m^-2·s^-1 and 126.95 ILμE·m^-2·s^-1） and two salinity levels （25 and 30）. The results showed that the best growth condition was at 20 ℃, 78.12 μE·m^-2·s^-1 of illumination, 0 of salinity and 8.3 of pH, for the maximum specific growth rate and the cell density were highest in this condition; the effect of temperature on algal growth was more significant than that of salinity, light intensity and pH, and the algal growth was restrained by other environmental factors referred in this experiment.