磷酰胆碱接枝涤纶人工血管的生物相容性

Biocompatibility of Dacron Vascular Prothesis Coated with 2-Methacryloyloxyethyl Phosphorylcholine in Animal Model

目的通过臭氧活化的方法在涤纶人工血管表面接枝2-甲基丙稀酰氧基乙基磷酰胆碱,进行接枝后人工血管血液相容性研究。方法通过臭氧活化的方法,在涤纶人工血管表面接枝2-甲基丙稀酰氧基乙基磷酰胆碱,接枝后的涤纶人工血管修剪后作兔腹主动脉补片(实验组),并和未接枝涤纶人工血管作对照。术后观察兔腹主动脉人工血管通畅率、吻合口狭窄程度、血流量和吻合口周围BrdU阳性细胞数。结果接枝2-甲基丙稀酰氧基乙基磷酰胆碱的涤纶人工血管补片吻合后,兔腹主动脉通畅率较对照组无明显差异;实验组兔腹主动脉吻合口狭窄程度小于对照组,而吻合口血流量大于对照组,吻合口周围BrdU阳性细胞数明显少于对照组,差异有统计学意义(P<0.05)。结论涤纶人工血管经接枝2-甲基丙稀酰氧基乙基磷酰胆碱后,能有效抑制吻合口内膜和平滑肌增生,减少吻合口狭窄程度,增加血流量。

Aim To study the biocompatibility of Dacron vascular prosthesis surface in rabbit model coated with 2-methacryloyloxyethyl phosphorylcholine（MPC） by ozonization.Methods Dacron vascular prosthesis was exposed to ozone and then followed by grafted polymerization with MPC.Pruned the vascular prothesis coated with MPC and patched the abdomenal aorta of twelve rabbits（treated group） controlled with uncoated grafts.The patency rate of abdomenal aorta,level of anastomotic stenosis,volume of blood flow of vascular prosthesis and the BrdU labeling index were observed.Results After patched the abdomenal aorta of rabbits using different Dacron vascular prosthesis,there was no significant difference in the patency rate between the two groups.Level of stenosis in anastomotic area was less in treated group than control group.The BrdU labeling index in neointimal tissue and smooth muscle around stoma was significantly less（P〈 0.05） in the treated group than in the control group.Conclusions These data demonstrated that Dacron vascular prosthesis coated with MPC significantly reduces anastomotic intima and smooth muscle hyperplasia,reduces level of anastomotic stenosis and increase volume of blood flow of vascular prosthesis.