急性早幼粒细胞白血病与MHC-A^＊基因的相关研究

MHC—A*gene polymorphism in patients with acute promyelocyti leukemia

目的探讨北方地区汉族人急性早幼粒细胞白血病(APL)与人类主要组织相容性复合体MHC—A*基因多态性的关系。方法应用顺序特异性引物和聚合酶链反应(PCR-SSP)技术,对21例急性早幼粒细胞白血病患者及29例无血缘关系的健康人的MHC—A*各等位基因及亚基因进行了检测分析。结果结果表明,MHC—A*11(RR=5·333,P<0·05=;和MHC—A*24(RR=3·60,P<0·05=基因与APL呈正相关,显著高于对照组,两组之间比较差异有显著性意义,而其它MHC—A*各等位基因未见异常,均无统计学差异。结论本项研究结果提示,MHC—A*11和24基因可能是我国北方汉族人APL致病的易感基因,为揭示APL的发病机制中免疫遗传学作用提供了重要信息和依据。

Objective： In order to study the correlativity between MHC-A ＊ gene polymorphism and acute promyelocyti leukemia （APE）. Methods： MHC-A ＊ gene polymorphism in 21 patients with APL and 29 normal control subjects was analyzed by using the polymerease chain reaction ／ sequence specific primer （ PCR ／ SSP） technique. Results ： MHC-A ＊ 11 （ RR =5,333, P 〈 0. 05 = ; MHC-A ＊ 24 （ RR = 3.60, P 〈 0. 05 =. The frequency of MHC - A ＊ 11 and 24 allele was significantly higher in patients than that in the controls. Conclusion： These findings further verify that immunogenetics play a predominant Pathogenetics role in a subset of APL patients. Individuals carrying MHC - A ＊ 11 and 24 alleles may be susceptibility gene to APL. This method is more specific, sensitive, simple, rapid and accurate, and could be applied to the routine clinical detective.