色素上皮衍生因子对视网膜光损伤保护作用的体内研究

Study of pigment epithelium derived factor protecting photoreceptors from light damage in vivo

目的：探讨色素上皮衍生因子（pigment epithelium derived factor，PEDF）对光损伤的视网膜感光细胞的作用。方法：Sprague Dawley（SD）鼠右眼玻璃体腔内注射PEDF，左眼注射磷酸盐缓冲液（phosphate buffered solution，PBS）作对照。注射2d后将SD鼠置于1000～14001x弥散白色冷荧光灯下连续光照2，5，7d后分别行ERG检查和组织学分析。结果：连续光照2，5，7d后，PEDF处理组与PBS对照组的外核层（outer nuclear layer，ONE）厚度及ERGb波振幅均呈逐渐下降趋势，统计学分析两组差别具有显著性意义（P〈0．01）。结论：PEDF对光损伤的视网膜感光细胞具有保护作用。

AIM： To explore the effect of pigment epitheliumderived factor （ PEDF） on the retinal photoreceptor cells of rats damaged by bright continuous light （BCL）. METHODS： Five to eight-week-old albino Sprague- Dawley （SD） rats received an intravitreal injection of PEDF in right eyes. Fellow eyes received phosphate- buffered saline （ PBS） and sewed as control group. Two days after the injection, the SD rats were exposed to the diffuse cool white light of 1000-14001x for different periods （2, 5, 7 days）. ERG responses were recorded after BCL damage. The ocular tissues were then processed for histological examinations. RESULTS： After 2, 5, 7 days＇ BCL exposure, the thickness of the outer nuclear layer （ONL） and ERG responses all decreased in two groups, and there were significant differences between the mice in control and PEDF treatment group. CONCLUSION ： PEDF protect photoreceptor cells function and morphology from BCL damage.