基质金属蛋白酶抑制剂GM-6001对大鼠角膜碱烧伤后新生血管的抑制作用

Restrained effect of GM-6001 the tissue inhibitor of matrix metalloproteinases on the growth of rat's corneal neovascularization after alkaline burn

目的:研究基质金属蛋白酶抑制剂GM-6001对大鼠角膜碱性烧伤后角膜新生血管的抑制作用。方法:取健康成年Wistar大鼠20只,随机分成实验组和对照组,每组10只。两组均建立大鼠角膜碱性烧伤模型。实验组滴用GM-6001滴眼液、氧氟沙星滴眼液和硫酸软骨素滴眼液;对照组滴用氧氟沙星滴眼液和硫酸软骨素滴眼液;各种滴眼液每日点眼4次,共7d。裂隙灯下观察角膜溃疡和角膜新生血管生长情况。结果:碱烧伤后实验组和对照组出现新生血管的时间无显著差异(P>0.05)。新生血管的生长长度与生长面积,在伤后1d和3d,实验组和对照组无显著差异(P>0.05);在伤后5d和7d,实验组和对照组有显著差异(P<0.05)。结论:基质金属蛋白酶抑制剂GM-6001点眼对角膜新生血管的生长有抑制作用。

AIM. To study the restrained effect of GM-6001 the tissue inhibitor of matrix metalloproteinases （TIMP） on the growth of rat＇s corneal neovascularization after alkalineburn. METHODS： Twenty Wistar rats were randomly divided into 2 groups, experimental group and control group, 10 rats in each group. In two groups, central corneas of rat eyes were burned by lmol/L NaOH for 120 seconds. GM- 6001 eyedrops, Ofloxacin eyedrops and chondroitin sulfate eyedrops were used in experimental group. Ofloxacin eyedrops and chondroitin sulfate eyedrops were used in control group. Eyedrops were applied four times a day for one week in each group. The occurrence and development of CNV were observed by slit-lamp microscope everyday. The length and the area of CNV were calculated. RESULTS; The occurrence of CNV in experimental group was （1.40 ±0.05）d , and was （1.25 ± 0.10）d in control, with no significant difference in the occurrence time of CNV（ P 〉 0.05 ）, in the length （ P 〉 0.05 ） and area of CNV at first day and third day after being burned. Significant difference（ P〈 0.05 ） was found at fifth day and seventh day after being burned. CONCLUSION ： GM-6001 restrains the progress of corneal ulcer and the growth of corneal neovascularization.