薄层层析法检测板蓝根所含靛玉红、靛蓝的方法研究

A Study on the Method of Indirubin and Indigo in Indigowoad Root by Thin-Layer Chromatograph

目的:对板蓝根所含靛玉红、靛蓝的薄层色谱进行实验性研究。方法:采用硅胶G板点样,选择最佳展开系统和样品最低检出量。结果:获得最佳色谱条件①硅胶G板为吸附剂;②石油醚-乙酸乙酯-氯仿(1∶1∶8)为展开剂。该色谱条件下,样品所含靛玉红、靛蓝的最低检出量分别为20μl、12μl。结论:本方法简便、快速、有效,可以作为板蓝根提取液靛玉红及靛蓝的相对含量的检测方法。

Objective： Thin-layer chromatography of indimbin and indigo in Indigowoad root has been studied. Methods： To select the best developer and the detection limits of the extracts of Indigowoad root on the silica gel G plates. Results The best experimental resuits could be showed on the silica gd G plates on condition that the developer was Ether-Ethyl acetate-Methyl alcohol （ 1 ： 1 ： 8） . The detection limits of indimbin and indigo in the samples were 20 and 12μl respectively. Conclusions： The method was simple, rapid and efficient. It can be used for the determine method of relative content of Indirubin and Indigo in the extracts of Indigowoad root.