拟除虫菊酯类农药多残留直接竞争ELISA建立及初步应用

Development of Direct Competitive ELISA for Pyrethriods and Its Application

以辣根过氧化物酶对兔抗拟除虫菊酯类农药抗体进行标记，通过优化分析条件和样品前处理方法，建立了可同时检测蔬菜中多种拟除虫菊酯类农药的直接竞争抑制ELISA方法（DC-ELISA），并在实际检测中进行了初步应用。该方法可用于同时检测蔬菜中甲氰菊酯、氯氰菊酯、三氟氯氰菊酯、溴氰菊酯4种农药的残留，检出限（I10）分别为0．25、0．30、0．43、0．81mg·L^-1，青菜样品添加含量为0．5-5．0mg·kg^-1，回收率分别为93％-114％，97％-110％，89％-126％，93％-113％。采用该方法对南京市场抽取的107个样品进行检测，并与气相色谱法进行比较，直接竞争ELISA阳性检出率为8．46％，气相色谱法阳性检出率为3．74％。

Based on labeling the antibody against pyrethriods with Horseradish Peroxidase（ HRP）, a direct competitive ELISA （ DC - ELISA） for fast screening of a group of pyrethroid pesticides was de- veloped. The detection limits of the residues of fenpropathrin, cypermetnrin, cyhalothrin and delta-methrin were 0. 25, 0. 30, 0. 43 and 0. 81mg·L^-1, respectively. The recovery ranges obtained by spiking the four pesticides at the levels of 0.5-5.0mg·L^-1 were 93% - 114% , 97% - 110% , 89%- 126% and 93%- 113% , respectively. Pyrethroid pesticides residues of 107 vegetable samples collected from the Nanjing markets were screened by the developed DC-ELISA, and validated by gas chromatographic （GC） detection. The positive detection rate was 8.46% for DC-ELISA, and 3.74% for GC detection.