小麦谷元粉中三聚氰胺的高效液相色谱法测定

Determination of Melamine in Wheat Gluten by High Performance Liquid Chromatography

小麦谷元粉中三聚氰胺经乙腈-水萃取、提纯后，采用Kromasil 100-5C。柱，以50％（体积分数）乙腈水溶液-庚烷磺酸钠（0．01mol／L，pH3、3）（体积比15：85）作流动相，该方法高、中、低3个浓度水平样品回收率为91％-96％；3个浓度梯度的相对标准偏差（n=8）为0．05％-10．2％；方法检出限（S／N=3）为65μg／L。

A method for the determination of melamine in wheat gluten by high performance liquid chromatography（HPLC） was developed. After being extracted by acetonitrile and water from samples melamine was separated on a Kromasil 100-5, 250mm×4.6 mm, Cs column with acetonitrile and sodium 1-heptanesulfonate（0.01 mol/L, pH 3.3, 15 ： 85, by volume） detected by ultraviolet detector. The average recoveries were 91% -96% for high, middle and low concentration levels（ n = 8 ） in this method. The relative standard deviations（RSD） were 0.05% - 10.2% （ n = 8） ; and the detection limit（ S/N = 3 ） was 65μg/L.