66例早期钩体病患者血和尿标本的聚合酶链反应及生物素分子杂交分析

Analysis on Speciems of Serum and Urine in 66 Cases of Early Leptospirosis by PCR and Biotin AMPPD Hybridization

采用国际通用引物Ｇ１Ｇ２对６６例发病在一周内钩体病患者的血和尿标本进行了ＰＣＲ检测及生物素－链霉抗生素蛋白碱性磷酸酶体系标记的重组ＤＮＡ探针杂交分析。结果表明，ＰＣＲ技术结合生物素分子杂交分析，不仅排除了非特异性扩增，增加了可靠性，同时也提高了检测的敏感性（从７１．３％升至８６．１％）；经统计学分析发现早期尿标本的ＰＣＲ检测阳性率与同期血标本的ＰＣＲ检测阳性率没有统计学差别，由于尿标本易于收集和处理，所以对尿标本进行ＰＣＲ检测对钩体病的早期诊断和后期监测更值得重视和推广。

We used polymerase chain reaction (PCR) and Biotin AMPPD hybridization to detect leptospiral DNA in blood and urine samples in 66 patients at the early stage of leptospirosis. The results showed that PCR and Biotin AMPPD hybridization not only ruled out the non specific PCR amplification and increased the reliability for clinical specineus of leptospirosis but also raised the detecting sensibility (from 71.3% to 86.1%). The positive rates of PCR resulted from serum cord urine showed no statistical difference; therefore urine sample is northy of application and dissemination for detecting leptospires at the early stage of leptospirosis. Urine sample is easier for one to collect, preserve and has less intervention. The primers G1,G2 are optimal opplication of PCR in epdemic areas in China.