摘要
用Amp-FLP技术分析D1S80位点的多态性,对85例亲子鉴定案和2例强奸杀人案进行了鉴定。在85例亲子鉴定案中,35例排除了错误被控父亲,50例未排除。在35例中,D1S80位点排除了30例,排除机率为35.29%,其中有2例只有D1S80位点排除了亲子关系;其余遗传标记排除33,排除机率为38.82%,有5例D1S80未排除。在50例未排除亲子关系的案例中,由D1S80位点得到的单项父权指数(PI)为2.0576~111.1111,在提高父权相对机会(RCP)上起了很大作用。50例中,41例的RCP达到了99.730%以上,肯定了父权,9例达到99.300%~99.649%,证明被控男子很可能是孩子生父。D1S80位点的个人鉴别能力高,在法医学亲子鉴定和个人识别中具有重要的作用。
This is a paper on paternity testing in 85 test cases and the identification of individuals from mixed stains of semen and vaginal secretion in two rape cases. We used primers flanking the hypervariable region of the D1S80 gene to amplify DNA extracted from fresh blood, blood stain, saliva stain, semen stain, mixed stain in vaginal swab, and from fetal tissues. The results revealed that 35 out of 85 alleged fathers in 85 paternity testing cases were excluded. In 30 of the 35 excluded cases, the alleged fathers were excluded by D1S80 locus analysis. The exclusion rate was 35.29%. In 2 of the excluded cases, the alleged fathers were excluded by D1S80 locus analysis only. In 50 nonexcluded paternity testing cases, the paternity index(PI) of D1S80 locus was 2.0576 111.1111. It indicates that D1S80 locus analysis plays an important role in elevation the relative chance of paternity (RCP) value. In 41 of the 50 nonexcluded cases, RCP was higher that 99.75%. RCP reached 99.300% 99.649% in 9 of cases. In one rape and homicide case, the genotype of D1S80 locus of sperm on the vaginal swab was detected. It gave a clue to investigation. In the other rape case, the genotype of D1S80 of sperm on the vaginal swab was consistent with the blood stain from the suspect. So the suspect was not excluded. Since the D1S80 locus has a high discrimination power it is very useful for forensic individual identification and parentage testing. Some problems have been discussed in this paper.
出处
《华西医科大学学报》
CSCD
1997年第2期154-159,共6页
Journal of West China University of Medical Sciences
基金
卫生部科学研究基金
纽约中华医学基金
关键词
D1S80位点多态性
PCR扩增
亲子鉴定
个人识别
D1S80 polymorphism Polymerase chain reaction(PCR) Individual identification Parentage testing