人类D1S80位点的Amp-FLP分析在法科学实践中的应用

The Application of D1S80 Locus Analysis to Forensic Problems by Using Amp FLP Technique

用Ａｍｐ－ＦＬＰ技术分析Ｄ１Ｓ８０位点的多态性，对８５例亲子鉴定案和２例强奸杀人案进行了鉴定。在８５例亲子鉴定案中，３５例排除了错误被控父亲，５０例未排除。在３５例中，Ｄ１Ｓ８０位点排除了３０例，排除机率为３５．２９％，其中有２例只有Ｄ１Ｓ８０位点排除了亲子关系；其余遗传标记排除３３，排除机率为３８．８２％，有５例Ｄ１Ｓ８０未排除。在５０例未排除亲子关系的案例中，由Ｄ１Ｓ８０位点得到的单项父权指数（ＰＩ）为２．０５７６～１１１．１１１１，在提高父权相对机会（ＲＣＰ）上起了很大作用。５０例中，４１例的ＲＣＰ达到了９９．７３０％以上，肯定了父权，９例达到９９．３００％～９９．６４９％，证明被控男子很可能是孩子生父。Ｄ１Ｓ８０位点的个人鉴别能力高，在法医学亲子鉴定和个人识别中具有重要的作用。

This is a paper on paternity testing in 85 test cases and the identification of individuals from mixed stains of semen and vaginal secretion in two rape cases. We used primers flanking the hypervariable region of the D1S80 gene to amplify DNA extracted from fresh blood, blood stain, saliva stain, semen stain, mixed stain in vaginal swab, and from fetal tissues. The results revealed that 35 out of 85 alleged fathers in 85 paternity testing cases were excluded. In 30 of the 35 excluded cases, the alleged fathers were excluded by D1S80 locus analysis. The exclusion rate was 35.29%. In 2 of the excluded cases, the alleged fathers were excluded by D1S80 locus analysis only. In 50 nonexcluded paternity testing cases, the paternity index(PI) of D1S80 locus was 2.0576 111.1111. It indicates that D1S80 locus analysis plays an important role in elevation the relative chance of paternity (RCP) value. In 41 of the 50 nonexcluded cases, RCP was higher that 99.75%. RCP reached 99.300% 99.649% in 9 of cases. In one rape and homicide case, the genotype of D1S80 locus of sperm on the vaginal swab was detected. It gave a clue to investigation. In the other rape case, the genotype of D1S80 of sperm on the vaginal swab was consistent with the blood stain from the suspect. So the suspect was not excluded. Since the D1S80 locus has a high discrimination power it is very useful for forensic individual identification and parentage testing. Some problems have been discussed in this paper.