朱砂莲的组织培养及培养物HPLC分析

Callus Formation of Aristolochia tuberosa and Determination of Aristolochic Acid of Callus by HPLC

进行朱砂莲幼嫩茎段的愈伤组织诱导及培养，继代培养基采用①ＭＳ＋２，４－Ｄ０．５ｍｇ／Ｌ＋Ｋｔ０．２ｍｇ／Ｌ；②ＭＳ＋ＮＡＡ０．５ｍｇ／Ｌ＋６ＢＡ０．５ｍｇ／Ｌ，培养基１上愈伤组织生长较好，但显微观察表明，细胞形态变异较大，多呈管状，并有巨型细胞产生；用ＨＰＬＣ法对愈伤组织与原植物块茎、茎、叶进行分析和比较。愈伤组织均具有合成马兜铃酸的能力，其ＨＰＬＣ图谱与原植物茎段的图谱较一致，而与叶和块茎有较大差异，继代培养基１上的培养物，其马兜铃酸Ａ的含量（０．２７‰）高于原植物茎中的含量（０．１５‰）。

Studies on the callus induction starting from the young stems of Aristolochia tuberosa on MS medium were reported and the morphological characteristics of cultured cells were described.The results of HPLC determination showed that some callus cultures had higher aristolochic acid content than their original plant organs,and the callus cultured in MS medium +2, 4 D 0.5mg/L produced more amounts of airstolochic acid(0.27% of dry callus weight) compared to the original plant organ(0.15‰ of dry weight).