应用PCR-SSCP技术检测妊娠滋养细胞肿瘤p53基因突变

DETECTION OF p53 GENE MUTATIONS IN HUMAN GESTATIONAL TROPHOBLASTIC NEOPLASIA BY POLYMERASE CHAIN REACTION SINGLE STRAND CONFORMATIONAL POLYMORPHISM TECHNIQUES

应用聚合酶链式反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）溴乙锭染色技术检测２０例葡萄胎及４例绒毛膜癌组织石腊标本ｐ５３基因第５和８外显子基因突变。结果显示：正常绒毛０／９，葡萄胎６／２０（３０％），绒毛膜癌３／４发生ｐ５３基因突变。提示ｐ５３基因突变是滋养细胞肿瘤形成和演进过程中的一个重要事件，葡萄胎应属于良性滋养细胞肿瘤。

Using polymerase chain reaction single strand conformational polymorphism (PCR SSCP) and EB staining technique, paraffin embeded sections of 20 hydatidiform mole and 4 choriocarcinoma were detected in the exons 5 and 8 of p53 gene. The results showed that mutations of p53 gene were 0/9 in the normal chorionic villi, 6/20 (30%) in hydatidiform mole and 3/4 in choriocarcinoma. This study suggests that mutations of p53 gene may be an important event in human gestational trophoblastic neoplastigenesis and its progression.