摘要
采用UV-2100紫外分光光度仪测定硫酸小诺霉素含量。将本品与衍生化试剂邻苯二甲醛(OPA)进行胺缩醛反应,生成具有特定波长的紫外吸收的衍生产物,进行硫酸小诺霉素含量测定方法的探讨,并与高效液相色谱法比较。结果表明本法操作简便、快速,且测定结果准确、重现性好。
An ultraviolet spectrophotometric method for determination of micromonicin (MCR) was developed by the reaction of MCR with o pathaldehyde (OPA) and obtained a derivative absorbing ultraviolet in special wave length. The comparison was made between this method and high performance liquid chromatography (HPLC). The results indicate that the method is simple, rapid, precise and reproducible.
出处
《湖南医科大学学报》
CSCD
1997年第4期363-365,共3页
Bulletin of Hunan Medical University
关键词
紫外分光光度法
硫酸小诺霉素
含量测定
antibiotics,aminoglycoside
spectrophotometry,ultraviolet
methods