白僵菌转化的一个新蒽醌苷类化合物(英文)

A New Anthraquinone Glycoside Biotransformed by Beauveria bassiana

目的:研究白僵菌对大黄酸葡萄糖甲基化的转化作用。方法:在白僵菌培养液中加入大黄酸底物,利用生长细胞对其进行生物转化。发酵液离心后用乙酸乙酯萃取,乙酸乙酯萃取部位通过各种色谱方法分离得到一个新化合物。运用氢谱碳谱以及二维核磁共振光谱鉴定结构。结果:转化产物为大黄酸糖基化衍生物,结构鉴定为7-O-(4′-甲氧基-β-D-葡萄糖)。结论:芦荟大黄素(1)是一个新化合物。

AIM： To study the rhein methylglucosidation biotransformed by Beauveria bassiana. METHODS： Rhein was biotransformed by Beauveria bassiana through growing culture. The fermentation broth was centrifuged and extracted with EtOAc, and then EtOAc extract was subjected to a series of chromatographic separation methods to afford a new compound. The structure of the new compound was elucidated by means of ^1H and ^13C NMR spectroscopic analysis, including 2D NMR technique. RESULTS： The product was identified as a glycosylated derivative of rhein, and the structure was elucidated to be 7-O-（4＇-methoxy- β-D-glucopyranoside） aloe-emodin （1）. CONCLUSION： The compound isolated is a new one.