SARS-COV功能受体hACE2mRNA在人股骨头中表达的检测

Detection of the mRNA expression of human angiotensin-converting enzyme 2 as a SARS coronavirus functional receptor in human femoral head

目的检测人股骨头负重区软骨下松质骨中血管紧张素转化酶2(ACE2)mRNA的有效表达,由此来初步推断重症急性呼吸综合征(SARS)中冠状病毒是否通过S蛋白介导引起骨坏死。方法取成人正常股骨头负重区软骨下松质骨、软骨、肺组织,以Humanβ-actin基因表达为对照,分别采用RT-PCR方法检测组织中ACE2mRNA的有效表达。结果在上述各种组织中,只有肺组织能检测到ACE2mRNA的有效表达,股骨头负重区软骨下松质骨、软骨均未能检测到ACE2mRNA的有效表达;3种组织均能检测到Humanβ-actinmRNA的有效表达。结论SARS冠状病毒不可能通过S蛋白直接作用于股骨头而引起骨坏死,为临床进一步研究SARS患者股骨头坏死的致病机制提供了线索。

Objective To investigate the mRNA expression of severe acute respiratory syndrome-associated coronavirus （SARS-COV） functional receptor, angioteusin-converting enzyme 2 （ACE2）, in human femoral head and conjunctiva, and explore the possible entry route of SARS-COV in human femoral head. Methods ACE2 mRNA in human femoral head was detected by nested RT-PCR with human βactin gene as the positive control. Results The mRNA of human βactin gene could be amplified efficiently in all the tissue samples. The mRNA of human ACE2 was expressed efficiently in the normal lung tissue, but not in the cartilage and cancellous bone under the weight-bearing area of the femoral head. Conclusion SARS-COV can not infect the femoral head tissue and lead to avascular necrosis of the femoral head directly by the spike glycoprotein, and mechanism of the virus for causing avascular necrosis needs further investigation.