摘要
目的探讨从蜂王浆中提取蜂王酸(10-HAD)的工艺。方法二次有机溶剂提取,二次调pH,用高效液相色谱法对10-HDA进行定性分析。结果蜂王浆中10-HDA的最佳提取条件为:蜂王浆水溶液pH 10,加入乙醚提取杂质,再调pH 2加入乙醚提取10-HDA。结论此法可从蜂王浆中高效提取10-HDA。
Objective To discuss the technique of extraction of 10-HDA from royal jelly. Methods The HPLC was used for the qualitative analysis of 10-HDA with two organic solvents used and pH adjusted twice. Results The best extraction condition of 10-HDA from the royal jelly was as follows： first adjusting the pH of the solution to 10, then adding ether for the extraction of impurity; secondly adjusting the pH to 2, then adding ether for the extraction of 10-HDA. Conclusion This method can be used for the high effective extraction of 10-HAD from royal jelly.
出处
《食品与药品》
CAS
2008年第2期30-32,共3页
Food and Drug
关键词
蜂王浆
蜂王酸
提取
高效液相色谱法
测定
royal jelly
10-hydroxy-2-decenoic acid
extract
high pressure liquid chromatography
determination
