取食蜂蜜对棉铃虫齿唇姬蜂体内主要代谢物质的影响

Effects of honey feeding on carbohydrate, lipid and protein metabolism in Campoletis chlorideae

为揭示食物在齿唇姬蜂Campoletis chlorideae Uchida生命中所起的作用,测定分析饱食蜂蜜和只取食清水的齿唇姬蜂体内糖类、脂肪和蛋白质随日龄增加的变化趋势。结果显示,取食蜂蜜对齿唇姬蜂体内主要代谢物质影响显著。10%蜂蜜水和清水饲养的齿唇姬蜂的雌蜂和雄蜂在刚羽化时体内都含有较高水平的糖原、总糖、脂肪和蛋白质。但随着日龄的增加,用清水饲养的雌、雄蜂体内的糖原和总糖含量迅速下降并达到最低水平;而用蜂蜜水饲养的雌、雄蜂随日龄的增加体内的糖原含量没有显著变化,并且与清水饲养的雌、雄蜂相比,其体内总糖含量反而有所上升。用蜂蜜水饲养的齿唇姬蜂的寿命显著长于用清水饲养的齿唇姬蜂。由于蜂蜜中含有丰富的果糖,所以冷蒽酮反应测定显示,用蜂蜜水饲养的齿唇姬蜂体内果糖含量较高,但却不能在清水饲养的齿唇姬蜂体内测出。2种食物处理的齿唇姬蜂在羽化后体内脂肪含量均有下降;并且随日龄的增加,清水饲养的齿唇姬蜂体内脂肪含量的下降速度明显快于蜂蜜水饲养的齿唇姬蜂。取食蜂蜜水的齿唇姬蜂体内蛋白质含量随日龄无显著变化;而用清水饲养的齿唇姬蜂在羽化后体内蛋白质含量随日龄的增长迅速下降并达到最低水平。

We compared carbohydrate, lipid and protein levels in starved and honey-fed adults of Campoletis chlorideae Uehida. The results showed that feeding 10% honey solution had significant effects on the metabolite concentrations in C. chorideae. Both males and females that were provided different diets （honey solution, or water only） emerged with relatively high reserves of glycogen, body sugar, lipid and protein. Along with age growth, glycogen and body sugar reserves declined rapidly in individuals fed on water and were nearly depleted in the end in both sexes. By contrast, adult C. chlorideae fed on 10% honey solution maintained body sugar levels at least as high as that when emergence, and lived significantly longer than individuals fed on water. In addition, the glycogen levels were maintained at high levels. Due to honey solution comprising abundant fructose, both males and females fed on 10% honey solution showed high fructose levels. However, the cold anthrone tests failed to detect fructose within unfed C. chlorideae. Lipid levels declined in both diet treatments, but in water-fed individuals they declined more rapidly than honey-fed individuals. Protein levels in C. chlorideae fed on honey solution didn＇t change greatly, but they declined rapidly in post-emergence water-fed individuals and were reduced to the least in the end.