降血压肽基因工程菌的构建及高效表达策略

Strategys of Recombinant Construction and High Expression for Antihypertensive Peptides

降血压肽具有很好的降血压效果而且天然无毒副作用,是目前基因工程研究的热点之一。阐述了降血压肽基因工程菌构建的重要性,综述了国内外关于降血压肽及各种小肽表达方面的研究进展。探讨了降血压肽基因工程菌在构建过程中目的片段的选择、串联连接的方式、串联体的获得及表达载体的选择等方面采用的策略和依据,重点介绍了各种不同表达类型载体的优缺点。旨在能对今后做降血压基因工程菌的多肽表达提供参考,以方便实验设计。

Antihypertensive peptides （AHPs） have good effect of decreasing blood pressure, also have low adverse effects and toxicity. Now it has been received great interests in gene engineering. The necessity of construction gene-engineering strain which expressing AHPs and other small peptides were reviewed. The strategys and theoretical foundation of chosen peptides, construction tandem repeats, chosen expression vectors during the gene engineering constructions, the advantages and disadvantages of different types of expressing vectors were discussed.