17-β雌二醇对人成骨样细胞系MG63Cbfa1表达的影响

Effect of 17-β estrodiol (E2) on Cbfa 1 expression in MG 63 cells

目的研究17-β雌二醇对人成骨肉瘤MG 63细胞株Cbfa 1表达的影响,了解雌激素预防骨质疏松的机制中有无Cbfa 1的参加。方法17-β雌二醇10-6mol/L、10-8mol/L、10-10mol/L浓度干预人成骨肉瘤MG 63细胞株,24、48h后抽提RNA、蛋白,半定量RT-PCR,Western blot检测Cbfa 1 mRNA、蛋白表达的变化。结果人成骨肉瘤MG 63细胞中有Cbfa 1 mRNA和蛋白的表达。17-β雌二醇10-6mol/L、10-8mol/L、10-10mol/L浓度作用24或48h,对MG 63细胞的Cbfa 1 mRNA和蛋白表达无影响。结论人成骨肉瘤MG 63细胞株中有Cbfa 1表达,17-β雌二醇对MG 63细胞Cbfa 1基因的表达无影响。

Objective To observe the effect of 17-β estrodiol （E2） on Cbfa 1 mRNA and protein expression in MG 63 cells.Methods 17-β estrodiol 10^-6 mol/L, 10^-8 mol/L, 10^-l0 mol/L were used to treat MG 63 cells for 24 and 48 hours, Cbfa 1 mRNA expression protein expression was measured by RT-PCR and Western blot respectively. Results There are expression of Cbfa 1 in MG 63 cells both in mRNA and protein level, different concentration of 17-β estrodiol treated MG 63 cells for 24 and 48 hours have no effect on the expression of Cbfa 1 in MG 63 cells.Conclnsions Cbfa 1 is expressed in MG 63 cells, 17-β estrodiol has no effect on the expression of Cbfa 1 in MG 63 cells.