人精子甘露糖受体参与诱导卵母细胞皮质颗粒反应

THE CORTICAL GRANULE REACTION OF OOCYTES INDUCED BY MANNOSE RECEPTOR OF HUMAN SPERMATOZOA

目的研究人精子甘露糖受体与卵母细胞皮质颗粒反应的关系。方法以亲和层析法纯化的人精子甘露糖受体(purified mannose receptor,pMR)作用去透明带金黄地鼠卵,继而用罗丹明偶联的兵豆凝集素(Tetramethylrhodam ine Isothiocyanate Labeled Lentil,TRITC-LCA)标记。通过荧光显微镜观察被标记的皮质颗粒及卵母细胞表面甘露糖基的变化情况。结果pMR作用卵母细胞后,卵母细胞内的皮质颗粒减少且细胞表面的甘露糖基数量增加。结论pMR作用于卵母细胞表面后,可触发皮质颗粒反应,并使皮质颗粒部分内容物转移到卵母细胞表面。

Objective To investigate the relationship between the purified mannose receptor （pMR） and the cortical granule reaction of oocytes. Methods The zona free hamster eggs were pre - incubated with pMR isolated from motile human sperms by mannose -agarose gel affinity chromatography. Tetramethylrhodamine Isothiocyanate Labeled Lentil （TRITC -LCA） was used to label the cortical granules （CGs） and surface mannose, and observed by fluorescence microscopy. Results After pre - incubation with pMR, the CGs of the oocyte decreased, but the mannose increased on the oolemma. Conclusion The results indicate that pMR can induce the cortical granule reaction, and the matrix of CGs might migrate to the surface of the oolemma after pMR pre - treatment.