脑缺血后大鼠神经元和星形胶质细胞P21^(cip1)表达的研究

THE EXPRESSION OF P21^(cip1) IN NEURONS AND ASTROCYTES OF RATS AFTER FOCAL CEREBRAL ISCHEMIA

目的研究大鼠局灶性脑缺血再灌注损伤后细胞周期蛋白依赖性激酶抑制因子P21cip1在神经元和星形胶质细胞的表达。方法建立大鼠大脑中动脉阻塞(MCAo)再灌注模型,应用流式细胞术检测各组MCAo再灌注后不同时期神经元和星形胶质细胞中的P21cip1的表达。结果缺血侧皮层区星形胶质细胞和神经元中的P21cip1的表达在再灌注3d、7d、14d后表达下调,与假手术组比较有显著性差异(P<0.05);神经元中的P21cip1的表达和星形胶质细胞中的P21cip1的表达无显著性差异(P>0.05)。结论局灶性脑缺血再灌注损伤后,缺血侧皮层区星形胶质细胞和神经元的p21cip1表达下调。

Objective To study the change of expression of P21^cip1 in astrocytes and neurons of rats after focal cerebral ischemia. Methods Ischemia was induced by temporary middle cerebral artery occlusion （MCAo） . The experimental rats suffered from MCAo were sacrificed on days 1, 3, 7 and 14 after reperfusion. The brain was taken and the P21^cip1 of astrocytes and neurons was detected by flow cytometry. Results Compared with that of the controls, the expression of P21^cip1 in astrocytes and neurons was remarkably down-regulated on the ischemic side of cerebral cortex after 3, 7 and 14 days of cerebral ischemia/reperfusion （ P 〈 0. 05 ） . The P21^cip1 expression showed no marked difference between astrocytes and neurons （ P 〉 0. 05） . Conclusion Astrocytes and neurons have a downregulation of expression of P21^cip1 after focal cerebral ischemia.