阴离子交换树脂固定化果胶酶研究

Studies on the immobilization of pectinase by anion-exchange resin

以阴离子交换树脂为载体、戊二醛为交联剂,对果胶酶进行固定化分析,探讨了温度、pH值、时间、加酶量、戊二醛浓度、交联温度、交联时间对果胶酶固定化效果的影响,同时对固定化果胶酶的酶学特性进行研究.结果表明,果胶酶最佳固定化条件为:温度为40℃,pH值为5.5,固定化6 h,加酶量为0.75 mL/g树脂,戊二醛体积分数为0.1%,交联温度为4℃,交联时间4 h.在此条件下,固定化果胶酶的酶活回收率可达到80%以上.酶学特性试验表明,固定化果胶酶最适温度为60℃,最适pH值为4.0,具有较好的操作稳定性.

Pectinase was immobilized on anion-exchange resin by cross-linkage of glutaraldehyde. Effect of temperature, pH, time, amount of the enzyme, concentration of glutaraldehyde, crosslinking temperature and time were studied for the impact of immobilized pectinase. Then, the characteristics of immobilized enzyme was studied. The optimum conditions for the immobilization were as follows ： 40℃, pH = 5.5, 6 h, 0.75 mL pectinase/g resin. The concentration of glutaraldehyde was 0.1%, crosslinking temperature and time were 4℃ and 4h respectively. Under the optimal conditions, the activity recovery of immobilized pectinase can reach to more than 80 %. The characteristics of immobilized enzyme were as follows： the optimum temperature and pH were 60℃ and 4.0 respectively. Moreover, the immobilized enzyme had a good operational stability.