替米沙坦对高糖高脂喂养大鼠内皮功能的影响

Effect of telmisartan on endothelial function of the rats fed a high-sucrose and high-fat diet

目的观察替米沙坦对高糖高脂喂养大鼠血管内皮功能的影响。方法30只SD大鼠随机分空白对照组、模型组和用药组,模型组和用药组给予高糖高脂饲料喂养,用药组同时给予替米沙坦灌胃给药10周。实验结束时取血测定一氧化氮(NO)、内皮型一氧化氮合酶(eNOS)含量,取大鼠主动脉弓及胸主动脉做电镜观察和HE染色光镜观察。结果模型组与空白对照组比较,NO明显降低(30.50±5.40 vs 39.34±7.34,P<0.01),eNOS明显降低(35.07±6.59 vs 47.07±6.56,P<0.01),光镜下可看到局部内膜增厚并向管腔突起,电镜下可观察到内膜破损、内皮细胞脱落;用药组与模型组比较,NO升高(37.42±5.75 vs 30.50±5.40,P<0.05),eNOS明显升高(44.81±7.00 vs 35.07±6.59,P<0.01),光镜下内膜增厚不明显,电镜下可观察到内膜完整、内皮细胞无脱落。结论替米沙坦对高糖高脂喂养大鼠血管内皮功能有保护作用。

Objective Observe the effect of telmisartan on endothelial function of the rats fed a high-sucrose and high-fat diet. Methods Thirty SD rats were randomly divided into control group, model group and drug group, rats of the model group and drug group were fed a high-sucrose and high-fat diet, rats of the drug group were treated with the intragastric infusin of telmisartan for ten weeks. At the end of the trial, sampling blood and measuring the concentrations of nitric oxide （NO）, total nitric oxide synthase （tNOS）, induced nitric oxide synthase（ iNOS）, endothelial nitric oxide synthase（eNOS）, thoraicic aorta were examined through routine HE staining, aortic arch were examined through Transmission Electron Microscopy. Results Compared with the control group, NO was significantly decreased （ 30.50± 5.40 vs 39.34±7.34,P 〈 0.01 ）, eNOS was significantly decreased （ 35.07 ± 6.59 vs 47.07± 6.56, P 〈 0.01 ）, the pathological changes can be obviously observed; compared with the model group, NO was increased （37.42±5.75 vs 30.50± 5.40, P 〈 0.05 ） , eNOS was significantly increased （ 44.81 ±7.00 vs 35.07 ± 6.59,P 〈0.01 ）, the pathological changes obviously slight. Conclusion Telmisartan can protect endothelial function of the rats fed a high-sucrose and high-fat diet.