HPLC法测定文冠果各部位中文冠果皂苷E的含量

HPLC method for determination of bunkanka saponins E from different parts of Xanthoceras sorbifolia Bunge

目的建立HPLC法测定文冠果各部位的文冠果皂苷E（bunkanka saponins E）的含量。方法采用重结晶分离纯化，波谱分析鉴定其结构；HPLC法测定其含量。用HypersilODS2柱（250mm×4．6mm，5μm），流动相为甲醇-水-磷酸（体积比为70．00：30．00：0．02），流速为1．0mL·min^-1，柱温为34℃，检测波长为210nm。结果文冠果皂苷E质量浓度在0．0098～0．1960g·L^-1内与峰面积成良好线性关系r=0．9999（n=6）；果壳、果柄、种皮和种仁中文冠果皂苷E的平均回收率及RSD值分别为100．4％、1．64％（n=6）；100．2％、2．72％（n=6）；100．2％、2．12％（n=6）；99．2％、2．28％（n=6）。结论该方法为文冠果不同药用部位（果壳、果柄和种皮）的质量控制提供实验依据。

Objective To obtain and develop a HPLC method for the determination of the content of bunkanka saponins E from Xanthoceras sorbifolia 13unge. Methods 13unkanka saponins E was isolated by chromatography and purified by recrystallization method. On the basis of spectral data, the structure was identified. A Hypersil ODS2 column （250.00 mm× 4.6 mm, 5 μm） was used with a mobile phase of methanol-waterphosphoric acid （ V： V： V = 70.00：30.00：0.02）,and the detecting wavelength was set at 210 nm, the col- umn temperature was 34℃. Results The calibration curve was linear over the range of 0.009 8 - 0.196 0 g·L^-1 with correlation coefficient of 0.999 9 （n = 6）. The average recoveries of bunkanka saponins E from the husk, the carpophore, the tunic and the kernel of Xanthoceras sorbifolia Bunge were 100.4% withRSD of 1.64% （n =6）,100.2% with RSD of 2.72% （n =6）,101.2% with RSD of 2.12 % （ n = 6）, 98.2 % with RSD of 2.28 % （ n = 6）, respectively. Conclusions The method is sensitive, accurate, rapid, and suitable for the quality control of the medicinal material.