期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

窖蛋白1在无血清状态下诱导FBJ细胞死亡 被引量：1

Caveolin-1 responsible for cell death of FBJ cell lines under serum-free conditions
下载PDF
导出
摘要 目的研究无血清状态下,FBJ-S1细胞较FBJ-LL细胞更易死亡的原因。方法用细胞活性检测法分析细胞的活性,分别用HPTLC法、逆转录PCR法和免疫印迹法检测细胞中GD1a和窖蛋白1(caveolin-1)的含量。结果研究表明,在无血清培养基培养条件下,富含神经节苷脂GM3和GD1a的低转移性的FBJ-S1小鼠骨肉瘤细胞趋向死亡,但对含有几乎相同量GM3而无GD1a表达的高转移性的FBJ-LL细胞的生长却未见明显影响。同时发现:a.FBJ-S1细胞富含窖蛋白1,而FBJ-LL细胞中窖蛋白1表达量则较低;b.利用小干扰基因沉默技术在FBJ-S1细胞中沉默窖蛋白1的表达,结果使细胞存活率升高;c.使用GD1a处理FBJ-LL细胞导致其趋向死亡,通过免疫印迹法检测窖蛋白1发现其蛋白表达量增加。结论在无血清培养条件下,窖蛋白1诱导FBJ细胞的死亡。 Objective To investigate the reason why FBJ-S1 but not FBJ-LL cells die in serum-free medium.Methods Cells viability was detected by WST assay. The expression of GDla and caveolin-1 （Carl） was monitored by HPTLC and RT-PCR or Western blots, respectively. Results Poorly metastatic mouse osteosarcoma FBJ-S1 cells expressing gangliosides GM3 and GDla underwent cell death, but highly metastatic FBJ-LL cells containing the same GM3 content though only very little GDla did not. In addition, 1） FBJ-S1 cells were rich in Carl compared to FBJ2 LL cells, 2） Cavl siRNA transfected S1 cells survived scrambled sequence transfected S1 cells, and 3） cultivation of FBJ-LL cells with GDla resulted in cell death in serum-free culture with elevated Carl expression as revealed by Western blotting. Conclusions Caveolin-1 is responsible for cell death under serum-free conditions.
作者 郑大勇 王毅楠 王丽 吴英良 山形贞子 山形达也
机构地区 沈阳药科大学生命科学与生物制药学院
出处 《沈阳药科大学学报》 CAS CSCD 北大核心 2008年第3期223-228,共6页 Journal of Shenyang Pharmaceutical University
关键词 神经节苷脂 GD1a 窖蛋白1 FBJ细胞 细胞死亡 ganglioside GD1a eaveolin- 1 FBJ cells cell death
分类号 R963 [医药卫生—微生物与生化药学]
  • 相关文献

参考文献13

  • 1HAKOMORI S, IGARASHI Y. Functional role of glycosphingolipids in cell recognition and signaling [ J ]. J Biochem. (Tokyo), 1995, 118 : 1091 - 1103.
  • 2HAKOMORI S. Tumor - associated glycolipid antigens, their metabolism and organization [J]. Chem Phys Lipids, 1986, 42:209 - 233.
  • 3HYUGA S, YAMAGATA S, TAI T, YAMAGATA T. Inhibition of highly metastatic FBJ - LL cell migration by ganglioside GDla highly expressed in .poorly metastatic FBJ - Sl cells [J]. Biochern Biophys Res Commun, 1997, 231:340 - 343.
  • 4HYUGA S, YAMAGATA S, TAKATSU Y, et al. Suppression of FBJ - LL cell adhesion to vitronectin by ganglioside GDla and loss of metastatic capacity[J]. International J Cancer, 1999, 83:685 -691.
  • 5WANG L, TAKAKU S, WANG P, et al. Gangfioside GDla regulation of caveolin - 1 and stiml expression in mouse. FBJ cells: Augmented expression of caveolin- 1 and Stiml in cells with increased GDla content [J]. Glycoconj J, 2006, 23 : 303 - 315.
  • 6HU D, TAN X, SATO T, . et al. Apparent suppression of MMP- 9 activity by GDla as deterrnined by gelatin zymography [J]. Biochern and Biophys Res Commun, 2006, 349:426 - 431.
  • 7YAMAGATA S, TANAKA R, ITO Y, et al. Gelatinase of murine metastatic tumor cells [J]. Biochem Biophys Res Commun, 1989, 158:228 - 234.
  • 8ISHIYAMA M, SHIGA M, SASAMOTO K, et al. A new sulfonated tetrazolium salt that produces a highly watersoluble formazan dye [ J ]. Chem Pharm Bull ( Tokyo ), 1993, 41:1118 - 1122.
  • 9ROZEN S, SKALETSKY H J. Primer3 on the WWW for general users and for biologist programmers [ M ]. Totowa, NJ: Humana Press, 2000 : 365 - 386.
  • 10BRATTON DL, FADOK VA, RICHTER DA, et al. Appearance of phosphatidylserine on apoptotic cells requires calcium- mediated nonspecific flip - flop and is enhanced by loss of the aminophospholipid translocase [ J ]. J Biol Chem, 1997,272.26 159-26 165.

同被引文献11

  • 1LOCKSLEY R M, KILLEEN N, LENARDO M J. The TNF and TNF receptor superfamilies: Integrating mammalian biology [J]. Cell, 2001,104 ( 4 ) : 487 - 501.
  • 2VAN HORSSEN R, TEN HAGEN T L, EGGEMONT A M. TNF-alpha in cancer treatment: Molecular insights, antitumor effects, and clinical utility [ J ]. Oncologist,2006,11 (4) : 397 - 408.
  • 3WU S,BOYER C M,WHITAKER R S,et al. Tumor necrosis factor alpha as an autocrine and paracrine growth factor for ovarian cancer: Monokine induction of tumor cell proliferation and tumor necrosis factor alpha expression [ J ]. Cancer Res, 1993, 53 ( 8 ) : 1939 - 1944.
  • 4GAIOTTI D, CHUNG J, IGLESIAS M, et al. Tumor necrosis factor-alpha promotes human papillomavirus (HPV) E6/E7 RNA expression and cyclin-dependent kinase activity in HPV-immortalized keratinocytes by a ras-dependent pathway [ J 3. Mol Carcinog, 2000127 (2) :97 - 109.
  • 5MAEDA M, WATANABE N, OKAMOTO T, et al. Endogenous tumor necrosis factor functions as a resistant factor against adriamycin [ J ]. Int J Cancer, 1994,58 (3) :376 - 379.
  • 6MURAKAMI A, KAWABATA K, KOSHIBA T, et al. Nitric oxide synthase is induced in tumor promotersensitive, but not tumor promoter-resistant, JB6 mouse epidermal ceils cocultured with interferon-gammastimulated RAW264.7 cells: The role of tumor necrosis factor-alpha [ J ]. Cancer Res, 2000, 60 ( 22 ) : 6326 - 6331.
  • 7ORTON R J, STURM O E, VYSHEMIRSKY V, et al. Computational modelling of the receptor-tyrosine- kinase-activated MAPK pathway [ J ]. Biochem J, 2005,392(2) :249-261.
  • 8RIVAS M A, CARNEVALE R P, PROIETTI C J, et al. TNF alpha acting on TNFR1 promotes breast cancer growth via p42/P44 MAPK, JNK, Akt and NF-kappa B-dependent pathways[J].Exp Cell Res,2008,314 (3) :509 -529.
  • 9WANG Li, WANG Yi-nan, SATO T, et al. Ganglioside GDla suppresses TNFα expression via Pknl at the transcriptional level in mouse osteosarcoma-delived FBJ cells [ J ]. Biochem Biophys Res Commun, 2008,371 (2) :230 - 235.
  • 10YAMAGATA S, MIWA M, TANAKA K, et al. FBJ virus-induced osteosarcoma has type V collagen consisting of A,B and C-like chains in addition to type I collagen [J]. Biochem Biophys Res Commun, 1982, 105(3) :1208 - 1214.

引证文献1

二级引证文献6

沈阳药科大学学报
2008年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部