单克隆抗体HIM_(82)对中性粒细胞呼吸爆发的降调节作用

Down-regulation Effect of HIM_(82) on Respiratory Burst of Neutrophil

本研究用单抗HIM82对中性粒细胞（PMN）呼吸爆发的早期调控，发现HIM82（30μg／ml）对佛波酯（PMA）激活的PMN产生的O2-、H2O2分别可下调至（44．00±8．9）％（n=8，P＜0．01）和（65．16±15.41）％（n=8，P＜0．01），对因呼吸爆发产生的活性氧物质（reactiveoxygenspecies，ROS）引起质膜中性内肽酶（NEP）的纯化作用（inactivation）[活性下降（43．29±9．41）％，n＝8，P＜0．01]有防护作用[活性恢复至（6648±15.53）％，n=8，P＜0．05]。结果表明该单抗对PMA激发PMN呼吸爆发产生O2-、H2O2有明显的降调节作用，也表明质膜上相应分化抗原分子对PMN功能特别是对激活呼吸爆发的机构有重要影响。

Objective The early control of respiratory burst of Neutrophil (PMN) was studied by using McAb HIM82. Methods PMNs were isolated from healthy volunteers. Superoxide anion, Hydrogen peroxide were measured in the presence of HIM82 FMLP,Sustance P and the NEP activities was measured in the presence of HIM82 alone. Results The amount of O2- and H2O2 produced in experimental group (by combining with HIM82) are (44. 00±8. 9) % (n = 8,P＜0. 01 ) and (65. 16±15. 41) % (n = 8,P＜0. 01 )of control group respectively. The activity of NEP inactivated by PMA (without HIM82) is (43. 29 ± 9. 41 ) % (n = 8,P＜0. 05)and the activity is (66. 48 ±15. 53) % (n = 8, P＜0．05) in PMA-inactivated group with HIM82. Conclusions The result mentioned above showed that the HIM82 monoclonal antibody exerts obvious down regulation on respiration burst induced by PMA and antagniation on inactivity of NEP. We under stand that this McAb has important regulation effect on function of PMN, it shows that the specific combination with corresponding antigen on membrane is essential for function of PMN especially for mechanism of respiratory burst of activated PMN.