一株小鼠自发性乳腺癌细胞系MA-891的建立及其免疫生物学特征

MA891──An Established Spontaneous Mouse Mammary Adenocarcinoma Cell Line and Its Immunologic Characteristics

从津白Ⅱ号小鼠自发性乳腺癌TA2MA891建立了体外长期传代细胞系MA891。MA891细胞保持了TA2MA891的高转移特性，皮下接种后肿瘤进行生长，并无一例外地发生肿瘤肺转移。MA891细胞的免疫原性极弱，表现为：（1）淋巴细胞-肿瘤细胞混合培养（MLTC）只能诱导出低水平的细胞毒T细胞（CTL）；（2）肿瘤浸润淋巴细胞（TIL）含量少，体外经IL－2扩增后只表现出微弱的细胞毒活性；（3）在小鼠体内不能诱导特异性肿瘤排斥反应。MA891可作为研究人类肿瘤生物治疗的有用实验模型。

Objective To characterize the immunologic properties of an established mouse mammary tumor cell line MA891． Methods From a spontaneous mouse mammary adenocarclonma（TA2 MA891） of TA2 mouse origin， an in vitro passaged cell line MA891 was maintained in RPMI164o with 10％ new born calf serum． The immunogenecity of MA891 was studied by classical tumor transplantation rejection assay， generation of cytotoxic T lymphocytes（CTL） by syngeneic secondary mixed lymphocyte－tumor cell culture（MLTC） and cytotoxic assayof tumor infiltrating lymphocytes（TIL）． Results MA891 maintained the high metastatic potential of the parental TA2 MA891 tumor passaged in vivo． Transplantation rejection studies indicated that amputation of a hind limb with growing tumor in the footpad did not protect the mice from a second subcutaneous challenge of the same tumor． The cytotoxic activities of both CTL and TIL were shownto be very weak．Conclusion MA891 is a mouse tumor of very low immunogenecity． It can be used as a mouse tumor model for studying cancer metastasis in humans．