摘要
为了了解慢性肝炎和肝癌病人患者体内乙型肝炎病毒(HBV)复制与HBV血清标志之间的关系,用酶联免疫吸附实验(ELISA)、聚合酶链反应(PCR)及斑点杂交方法对61例慢性肝炎和47例肝癌患者的HBV表面抗原(HBsAg)、相关e抗原(HBeAg)、表面抗体(抗-HBs)、核心抗体(抗-HBc)、相关e抗体(抗-HBe)进行了检测。结果表明:HBVDNA在HBsAg、HBeAg、/抗-HBc阳性的慢性肝炎和肝癌患者血清中的检出率分别为90.50%和50.00%;在HBsAg/抗-HBe、抗-HBc阳性者的检出率分别为45.40%和7.14%;在HBsAg阳性、HBeAg阴性/抗-HBe阴性者中的检出率分别为60.00%和40.00%;HBsAg阴性、/抗-HBc阳性或/抗-HBe阳性或/抗-HBs阳性者中的检出率分别为20.00%和22.22%;在血清学指标全阴性时,慢性肝炎和肝癌患者血清中HBVDNA的检出率均为0。实验提示:无论是肝炎或肝癌,在HBsAg、HBeAg同时阳性时,HBV复制最为活跃;在单独HBsAg阳性时,HBV有一定程度的复制;HBV复制在肝癌细胞中受到一定程度的抑制。
HBV immunological detection and HBV DNA detection by polymerase chain reaction (PCR) or molecular hybridization with 32 P labeled probe were done in 61 patients with chronic hepatitis (CH) and 47 patients with hepatocellular carcinoma (HCC) to investigate the relationship between HBV replication and serum markers. The HBV DNA was detected in 90.50% of CH patients and 50.00% of HCC patients with HBsAg, HBeAg and anti-HBc positive; in 45.40% of CH patients and 7.14% of HCC patients with HBsAg, anti-HBe and anti-HBc positive; in 60.00% of CH patients and 40.00% of HCC patients with HBsAg and negative HBeAg, anti-HBe positive; in 20.00% of CH patients and 22.22% of HCC patients with HBsAg negative and anti-HBc or anti-HBe or anti-HBs positive; in 0 of CH or HCC patients lack of HBV serum markers. Our data suggested that the most active HBV replication was correlated to the presence of both HBsAg and HBeAg, there were some extent of HBV replication in CH or HCC patients with positive HBsAg, the inhibition of HBV replication was related to anti-HBe, the less active HBV replication was seen in HCC patients when compared with CH patients.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1997年第3期220-222,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
肝炎
病毒学
肝癌
聚合酶链反应
斑点杂交
Hepatitis/Virology Hepatocellular carcinoma/Virology Polymerase chain reaction Dot blot hybridization Enzyme linked immunosorbent assay