PPARγ,、TLR4基因多态性与2型糖尿病的相关性研究

The Relationship between PPAR γ and TLR4 Gene Polymorphisms and Type 2 Diabetes Mellitus

目的:探讨天津地区人群中过氧化物酶体增殖物激活受体γ(PPARγ)基因Pro12Ala及toll样受体4(TLR4)基因Asp299Gly、Thr399Ile的多态性与2型糖尿病(T2DM)的关系。方法:提取365例T2DM患者(T2DM组)和95例健康人(对照组)的基因组DNA,PCR扩增目的片段后,用变性高效液相色谱技术(DHPLC)筛查基因突变情况。结果:T2DM组和对照组PPARγ基因Pro12Ala位点P/A的基因型频率分别为0.0740和0.0316(27/365和3/95),差异无统计学意义(P>0.05);两组均未筛查到TLR4基因Asp299Gly、Thr399Ile突变基因型。结论:天津地区人群PPARγ基因Pro12Ala、TLR4基因Asp299Gly、Thr399Ile多态性与T2DM无明显关联。

Objective： To explore the relationship among peroxisome proliferator-activated receptorγ （PPARγ） gene Pro12Ala and toll-like receptor 4（TLR4） gene Asp299Gly, Thr399Ile polymorphisms and type 2 diabetes mellitus （T2DM） in Tianjin area. Methods： To extract the genome DNA from 365 patients with T2DM and 95 healthy controls,and use polymerase chain reaction （PCR） to amply the objective fragment,then use DHPLC to detect the mutation. Results： There were no significant differences in genotype frequencies （P/A） of PPARγ gene Prol2Ala polymorphism between T2DM and control groups （0.074 0,27/365 and 0.031 6,3/95, respectively,P 〉 0.05 ）. The genotypes of TLR4 gene Asp299Gly and Thr399Ile mutations were not found in the two groups. Conclusion： There were no significant correlation among PPARγgene Prol2Ala,TLR4 gene Asp299Gly,Thr399Ile polymorphisms and type 2 diabetes mellitus in Tianjin area.