肝细胞生长因子和c—Met在胃癌组织中的表达及其意义

The expression of HGF and c-Met in gastric carcinoma

目的分析肝细胞生长因子（HGF）和c—Met与胃癌生物学行为和预后的关系。方法采用HGF、c-Met、Lyve-1和CD34对58例胃癌组织作免疫组织化学染色并计数，结合胃癌的临床病理资料作相关统计学分析。结果胃癌组织中HGF和c—Met表达明显上调，其微血管密度（26±10）和微淋巴管密度（24±9）均高于胃溃疡组织[（20±4）、（12±4）]，差异均有统计学意义（P〈0．01）；有淋巴转移的胃癌组织中HGF和c—Met的阳性表达率分别为86％和80％，高于无淋巴转移的胃癌组织（57％、36％），差异均有统计学意义（P〈0．05）；有远处转移的胃癌组织中HGF和c-Met阳性表达率分别为100％和94％，高于无远处转移的胃癌组织（71％、60％），差异均有统计学意义（P〈0．05）；HGF和c-Met表达与肿瘤是否有淋巴转移、远处转移和微血管密度、微淋巴管密度之间有明显的正相关关系（P〈0．01）；HGF、c—Met阳性和阴性组的生存率之间比较差异有统计学意义（P=0．09、P〈0．01）；肿瘤侵袭深度、有无淋巴转移、有无远处转移、胃癌的HGF、c-Met表达和微血管密度、微淋巴管密度均是影响胃癌预后的独立因素（P〈0．05）。结论HGF和c-Met与胃癌的发生发展和预后密切相关，可以作为反映胃癌生物学行为和判断预后的有效指标。

Objective To investigate the relationship between HGF/c-Met and biological behavior and prognosis of gastric carcinoma. Methods The expression of HGF, c-Met, microvessel density and microlymphatic density in 58 cases of gastric carcinoma and 27 cases of gastric ulcer were evaluated by immunohistochemistry, using monoclonal antibodies for HGF, c-Met , CD34 and Lyve-I respectively. Results HGF and c-Met protein were overexpressed in gastric carcinoma, and were significantly higher than those in the mucosa of gastric ulcer （P 〈0. 01 ）. The microvessel density and microlymphatic density of gastric carcinoma were significantly different between those with and those without lymphatic metastasis and distant metastasis （ all P 〈 0. 05 ） ; The positive expression of HGF and c-Met in those with lymph node metastasis （86%, 80% ） was significantly higher than those without lymph node metastasis （57%, 36% ）, P〈0.05. That with distant metastasis （ 100%, 94% ） was higher than that without （71%, 60% ） ,P 〈 0. 01. The 5 year survival rate in patients with positive HGF and c-Met expression was much lower than that with negative expression（ P = 0. 019, P 〈 0. 01 ）, Multivariate analysis indicated HGF and c-Met expression, depth of invasion, lymph node metastasis, distant metastasis, microvessel density and microlymphatic density were all independent prognostic factors of gastric carcinoma. Conclusions Expression of HGF and c-Met closely correlated with carcinogenesis, development and prognosis of gastric carcinoma.