蓖麻蚕核糖体大亚基RNA基因3'-端序列分析及进化研究

Primary and Seco9ndary Structure of 3'-end of the Large Subunit Ribosomal RNA of Silkworm Attacus ricini and Evolution Implications as Inferred from the Gene Sequence

测定了蓖麻蚕核糖体大亚基ＲＮＡ编码区３’－端ＤＮＡ序列，分析了其二级结构，并与昆虫伊蚊、果蝇；线虫；脊椎动物人、小鼠、爪蟾；低等脊索动物海鞘以及真菌酵母、毛霉相应的保守区段进行了同源比较。邻接法分析表明，昆虫核糖体大亚基ＲＮＡ在进化上与５ＳｒＲＮＡ相似，有加快的趋势。

The DNA sequence of the 3' -end of LSu-rRNA coding region of silkworm .Attacus ricini was determined. By -comparisons with the corresponding sequence of H. sapiens, X.leavis,H momus, A. albopictus , D. melanogaster,C.elegants, S. pombe, M musculus and M racemosus, we found that the coding sequence we obtained is very conserved. Phylogenetic tree inferred by Neighbor-jioning Method showed that the rate of evolution of insects was much faster than vertebrate. In addition, the secondary structure has also worked out it is highly conserved.