用基因枪法将人工雄性不育基因导入小麦的研究初报

Preliminary Study of Transferring Engineered Male Sterile Gene intoWheat via Microprojectile Bombardment

利用ＰＤＳ１０００／氦气基因枪将人工构建的雄性不育基因（ＴＡ２９－Ｂａｒｎａｓｅ基因）导入小麦栽培品种豫责１８号的幼胚细胞。然后在含有１０～２０ｍｇ／Ｌ除草剂Ｂａｓｔａ的培养基础上筛选与分化。从１７０个幼胚中获得６株绿苗，对照的７０个幼胚中未得到绿苗。对其中３株已生根且长势好的绿苗进行Ｓｏｕｔｈｅｍ杂交分析，结果表明，这３株绿苗皆为转基因植株，转化效率达１．８％。

Immature embryos of a cultivar Yumai No. 18 of wheat (Triticum aestivum L.) were bombarded using PDS 1 000/ He particle delivery system in the presence of plasmid pTABNBAR 55 which contained a TA29-Barnase gene and seletable marker Bar gene. Resistant calli were selected on Basta (5 to 1 0mg/ L) containing medium. Six green plants were regenerated from resistant calli derived from 1 70 bombarded immature embryos. No green plant was regenerated from calli of 70 non-transformed embryos. Southern blot of 3 well-developed green plants confirmed stable integration of Barnase gene into wheat genome. The transformation frequency was 1 .8% .