APM对小麦根尖分生组织细胞异常有丝分裂的诱导

INDUCTION OF ABNORMAL CELL MITOTIS OF ROOT MERISTEMS OF TRITICUM AESTIVUM BY AMIPROPHOS METHYL

探讨了不同浓度（c／μmol．L－1）和不同处理时间（t／h）下APM对小麦根尖分生组织细胞有丝分裂的影响．实验结果表明，在c（APM）＝1～4μmol／L、处理t＜5h可明显提高有丝分裂指数，对前期和中期细胞分裂的促进作用更为明显；但c（APM）的增加与有丝分裂指数的提高无明显线型关系；APM可促使间期细胞微核形成、中期细胞染色体聚合现象和扰乱纺锤丝的功能．纺锤丝功能上的扰乱可引起细胞的“不均等分裂”和“多极化分裂”形成上述两种细胞异常有丝分裂的临界浓度和处理时间分别为c＝5μmol／L和t＞5h．

The effect of Amiprophos Methyl (APM) on cell mitotis of root meristems of Triticum aestivum was investigated at different concentrations (1~4 μmol/L) and treatment times (4~6 h). The results indicatedthat mitotic index could be obviously increased when root tip was treated at 1~4 μmol/L APM for 5 h. Therise of mitotic index had no linear relation with the increase of c(APM). APM could induce micro-nucleatein interphase cells, and made chromosome polymerized and spindle function disturbed in metaphase cells.The disturbance in spindle function could cause the unequal cell division and multipolar cell division.