紫外分光光度法测定射干中总异黄酮的含量

Determination of total isoflavones in Rhizoma Belamcandae. by UV spectrophotometry

目的：建立射干药材中总异黄酮的含量测定方法,为控制药材的内在质量提供依据。方法：采用紫外分光光度法对射干药材中的总异黄酮含量测定,测定波长为266nm。结果：射干苷浓度在1-9μg/mL范围内与吸收度呈良好的线性关系（r=0.9998,n=5）,平均加样回收率为98.52%（RSD=1.9%,n=5）。结论：该方法准确,简便,重现性好,可用作测定射干中总异黄酮的含量的方法。

Objective： To establish a method for the assaying of total isoflavonoes in the ultrasonic wave＇s extracts of Rhizoma Belamcandae and to provide basis for the study of its internal quality. Method： The contents of total flavonoes in Belameanda were determined by UV spectrophotometry with a detection wavelength at 266nm. Results： Good linear relationship with absorbability occurred when the concentration range of tectoridin was 1 - 9μ g,/mL （ r = 0.9998, n = 5 ）and its average recovery was 98.52% （ RSD = 1.9%, n = 5 ）. Conclusion ： The method is simple, quick and accurate,of the testing samples ,and can be used to determine the content of total flavonoes in Rhizoma Belamcandae.