摘要
为了深入探讨性分化分子机制,构建了小鼠基因文库以SRY探针筛选,分离到了两组阳性克隆,一组含EcoRⅠ/3.5kb,此片段中载有小鼠Y染色体上的Sry基因.另一组含EcoRⅠ/3.0kb或SalⅠ/3.0kb。
An EcoRⅠor SalⅠ3.0 kb fragment and EcoRⅠ3.5 kb fragment from the positive clones screened by SRY probe in male mouse genomic library and forced cloning library were described respectively.The 3.0 kb fragment containing SRY box is located outside of Y chromosome mamely the Sox gene and the Sry gene of Kun Ming white mice is included in the 3.5kb fragment.