摘要
degQ基因编码一个由46个氨基酸组成的多肽，能增强许多芽孢杆菌胞外酶基因的表达．以pMK4作克隆载体构建短小芽孢杆菌基因文库，并用DNA探针原位杂交法从中钓出degQ基因．对克隆基因的DNA序列进行了分析并证明克隆的短小芽孢杆菌degQ基因具有增强枯草杆菌蛋白酶和果聚糖蔗糖酶基因表达的能力．degQ基因克隆有助于研究芽孢杆菌的正调控机理并可望提高外源基因在芽孢杆菌中表达．
degQ is a positive regulator encoding a polypeptide of 46 amino acids and able to enhance the expression of many extracellular enzyme genes of Bacilli. B. pumilus genomic library was constructed using plasmid pMK4 as vector and degQ gene was screened from the library by in situ hybridization with DNA probe. The cloned gene was sequenced and shown to be capable of enhancing the expression of proteases and levansucrase genes of B. subtilis. Cloning of degQ gene is helpful in studying the mechanism of positive regulation and increasing the foreign gene expression in Bacilli.
基金
国家自然科学基金!39270011
