摘要
以Tbm-3（icl-，异柠檬酸裂解酶活力丧失的生化突变株）为出发菌株，经紫外线诱变，通过依据生化代谢所设计的选择培养基（L-阿拉伯糖平板与D-葡萄糖酸钠平板）对接的筛选方法，获得磷酸葡萄糖酸脱氢酶（PGDH，E．C．4．2．1．12）渗漏突变型（pgdh1）的生化突变型菌株Tbm3-18．该菌株经摇瓶发酵试验显示，比出发菌株Tbm-3提高产酸率8．9％和转化率8．1％．表明pgdh-或pgdh1生化突变型菌株的选育，对谷氨酸的积累是有利的，该选育生化模式是成功的．
The mutant Tbm3-18 with pgdh1 (the phosphogluconic acid dehydrogenase, E. C. 4. 2. 1.12,leakage) as biochemical genetic marker was obtained directly from the parent strain Tbm-3 (icl-) by selective breeding according to a biochemical screening type (inoculated separately on L-arabinose and sodium D-gluconate plating culture medium)after mutagenesis by ultraviolet ray.The L-Gln level accumulated and L-Gln/glucose using Tbm3-18 was increased about 8.9% and 8. 1% against the case using Tbm-3. The experiments proved that screening of biochemical mutant pgdh- or pgdh1 was an advantageing for effective accumulation of L-Glu.
关键词
谷氨酸
发酵
诱变育种
生化缺陷型菌株
PGDH
L-Gln fermentation, Mutation breeding, Phosphogluconic acid dehydrogenase,Biochemical defecitive mutant
