癌患者血清中癌细胞凝集因子特性的研究

Studies on the Characteristics of Agglutinating Factory in the Sera of Patients with Cancer

前曾报道癌患者血清中存在选择性凝集具高转移潜能的人鼻咽癌细胞的凝集因子．现除扩大了检测例数外，重点对癌患者血清中凝集因子的化学本质及生物学特性进行了系统研究，证明其可能是一种内源性凝集素（糖结合蛋白质），特异性识别甘露糖及β1，6分枝的N-乙酰氨基乳糖．其作用不依赖于Ca+，而还原剂可提高其活性并对高碘酸氧化敏感．对这种内源性凝集素在癌栓形成及癌细胞转移中的作用进行了讨论．

Metastasis is the most threatening characteristics of malignant tumors, and a very complicated process with multisteps. Cancer embolus formation is a pivotal event in cancer metastasis cascade. Highly metastatic cancer cells tend to agglutinate spontaneously in vivo and mediated by lectins in vitro. In order to explore whether there are some endogenous lectins that cause cancer cell agglutination, agglutinating activity of cancer patient's serum was measured with highly and low metastatic cancer cell lines, respectively. It had been found and reported previously that some factors which induce specifically metastatic human cancer cells (U) to aggregate existed in most sera of cancer patients, the characteristics of the agglutinating factor were studied. A number of evidence suggested that the agglutinating factor is of protein, and may be a kind of endogenous lectin with specificity for mannose and β1,6 branched N-acetyllactosamine agglutinating activity of the lectin was independent on Ca(2+), but enhanced by exogenous reductant in 30% cases.The activity was lost after periodate oxidation.