HPLC-荧光法测定替米沙坦血药浓度的方法学考察

Determination of telmisartan in human plasma by HPLC-fluorescence

目的:采用HPLC-荧光法测定替米沙坦的血浆药物浓度。方法:采用Agillent Zorbax SB-C18色谱柱(150mm×4.6mm,5μm),流动相为水-乙腈(58∶42,H3PO4调pH为4～5),流速1.0ml/min,荧光激发波长300nm,发射波长380nm。结果:替米沙坦浓度在5～200ng/ml范围内与峰面积具有良好的线性关系,最低检测限为5ng/ml。结论:该法准确可靠,适用于替米沙坦的血药浓度测定。

Objective：To determine the concentration of telmisartan in human plasma by HPLC-fluorescence method. Methods：Separation was achieved on Agillent Zorbax SB-CI8 column （ 150 mmx4.6 mm,5 μm）.The mobile phase consisted of water-acetonitrile（58：42, pH4-5）,with a flow rate of 1.0 ml/min,and detection with a fluorescence detector set at an exci-tation wavelength of 300 nm and emission wavelength of 380 nm. Results：A linearity of telmisartan concentration curve was obtained in a range of 5-200 ng/ml.The detection limit was 5 ng/ml.Conclusion：This method is accepted to determine the concentration of telmisartan.