婴幼儿腹泻的病毒学检测分析

Analysis and determination of viral infection in infantile diarrhea

目的:了解婴幼儿秋冬季腹泻中轮状病毒、星状病毒及肠道腺病毒的感染情况。方法:对2006年10月至2007年3月的182例急性水样腹泻婴幼儿的粪便标本,采用金标快速试剂盒检测轮状病毒抗原;采用酶免疫吸附测定法(EIA)检测星状病毒抗原及肠道腺病毒抗原。结果:182例标本中轮状病毒检出率35.2%(64/182);星状病毒检出率5.5%(10/182),4例合并轮状病毒感染;肠道腺病毒检出率3.3%(6/182),2例合并轮状病毒感染。结论:轮状病毒仍是婴幼儿秋冬季腹泻最主要致病原;星状病毒感染是婴幼儿病毒性腹泻的第二位病因;有混合性病毒感染发生。

Objective：To analyze the infection of rotavirus,astrovirus,enteral adenorirus in infantile diarrhea. Methods：From October, 2006 to March, 2007, a total of 182 children under 5 years of age hospitalized for acute watery diarrhea were enrolled in this study. Stool specimen was collected from each patient. The method of gold colloid device was used to detect rotavirus angiten. The specimins were tested for astrovirus angiten and enteral adenorirus angiten by using enzyme linked immunosorbent assay （ELISA）. Results：Among 182 cases,the detection rate of rotavirus angiten was 35.2%（64/182）, and the astrovirus angiten was 5.5%（10/ 182） ,Four samples were infected with rotavirus and astrovirus at the same time. The detection rate of enteral adenorirus angiten 3.3% （6/182）, and 2 samples were infected with rotavirus and enteral adenorirus. Conclusion： Rotavims is still the major etiologic agents of acute infantile diarrhea in winter. Astrovirus is the second important agent of viral diarrhea in infants, and mixed infection still exist.