缓释骨形态发生蛋白-2的壳聚糖温敏凝胶促进牙周组织再生的实验研究

A study on the effect of the chitosan thermosensitive hydrogel loading recombinant human bone mor-phogenetic protein-2 on repairing periodontal defects

目的观察自制的加载重组人骨形态发生蛋白-2(rhBMP-2)的壳聚糖温敏凝胶修复牙周组织缺损的效果。方法选取3只健康雄性杂种犬制备前磨牙区人工Ⅱ度根分叉区组织缺损模型,随机分为空白对照组、空白凝胶组及载药凝胶组,术中先严密缝合组织瓣,然后对空白凝胶组及载药凝胶组区域分别注射先期配制并消毒好的空白凝胶和载药凝胶,术后8周取材行大体及组织学观察。结果载药凝胶组出现明显的牙周组织再生,而空白对照组和空白凝胶组仅有少量的牙周组织再生。载药凝胶组与空白对照组及空白凝胶组均有统计学差异(P<0.05),空白对照组和空白凝胶组相比,没有统计学差异。结论载rhBMP-2的壳聚糖温敏凝胶可有效促进牙周组织再生,同时简化手术操作,是一项有潜力的牙周组织再生手段。

Objective To observe the effect of the chitosan thermosensitive hydrogel loading recombinant human bone morphogenetic protein-2 （rhBMP-2） on repairing periodontal defects. Methods To prepare artificial furcation defects model in the posterior area in 3 healthy male dogs, and then to inject chitosan thermosensitive hydrogel loading of rhBMP-2 after fast suturing tissue flap. The groups filled with nothing or filled only with chitosan thermosen- sitive hydrogel were the controls. The dogs were sacrificed after 8 weeks and the periodontal regeneration was observed histologically. Results The histological observation showed that the chitosan thermosensitive hydrogel loading rhBMP-2 group achieved apparent periodontal tissue regeneration occupying the majority of the defects and the control groups got only a small amount of periodontal tissue regeneration. Condusion The chitosan thermosensitive hydrogel loading rhBMP-2 can effectively promote the periodontal tissue regeneration, while simplifying the surgical operation. It might be a potential means for periodontal regeneration.