复发性阿弗他溃疡患者唾液中表皮生长因子和病损区表皮生长因子受体的定量研究

Quantity research on epidermal growth factor in saliva and epidermal growth factor receptor in biopsy samples of recurrent aphthous ulcer patients

目的探讨复发性阿弗他溃疡(RAU)患者溃疡期和间隙期唾液表皮生长因子(EGF)质量浓度的变化以及病损区表皮生长因子受体(EGFR)的表达有无缺陷。方法选择27例在溃疡期和间隙期均成功获取唾液样本的轻型RAU患者作为RAU1组,采集33例正常人的唾液样本作为对照1组,采用酶联免疫吸附法检测两组唾液样本中EGF质量浓度。另外选择31例轻型RAU溃疡期患者在溃疡基底部剪取小块组织样本作为RAU2组,采集35例无RAU者的正常黏膜组织作为对照2组,采用荧光定量逆转录聚合酶链反应检测两组组织样本内EGFR的RNA表达情况。结果RAU1组溃疡期患者唾液EGF质量浓度高于RAU1组间隙期和对照1组,而间隙期EGF质量浓度低于对照1组(P<0.05)。RAU1组和对照1组唾液EGF质量浓度在不同性别间差别不大,与年龄也没有相关性(P>0.05)。RAU2组EGFR的RNA表达强度高于对照2组,两组间有统计学差异(P<0.05)。结论RAU患者口腔溃疡的复发性可能与间隙期唾液EGF质量浓度减少有关;口腔溃疡的自愈性可能与溃疡期唾液EGF质量浓度增加和溃疡区EGFR表达增加有关。

Objective To examine the change of epidermal growth factor CEGF） concentration in saliva of recurrent aphthons ulcer（RAU） patients during the ulcerous and interval period and epidermal growth factor receptor （EGFR） in ulcer biopsy samples. Methods EGF data of the samples, which were 27 saliva samples from RAU gained not only in the ulcerous period but also in interval period and 33 ones from normal persons, were acquired through enzyme linked immunosorbent assay CELISA） and EGF standard curve. EGFR-RNA date of RAU biopsies, which were 31 biopsy samples from RAU got during the ulcerous period and 35 ones from normal persons, were surveyed by QF-RT-PCR. All RAU samples were obtained under the same level, which were the whole patients were minor aphthons ulcers and their ulcers occurred not over the first four days. All patients and normal persons were selected seriously under the rule of physical situations without any other diseases and histories of using medicines. Results The EGF concentration of saliva in RAU group at ulcer occurrence was higher than that in the interval period and the normal control with a significant test （F=3.24, P〈0.05）. The EGF concentration of saliva in RAU group during the interval period was lower than that in the control, which was significant on statistics （t=2.73, P〈0.05）. The EGFR-RNA in RAU group at ulcer occurrence was higher than the normal control with a significant test （t=3.15, P〈0.05）. Conclusion It was suggested that the ulcer occasion of RAU patients could be related with the decreas- ing of EGF in saliva during interval period, and that the ulcer self-cure of RAU patients would be contributed to the increasing of EGF in saliva and EGFR in ulcer tissues during ulcer occurrence.