消瘀化痰饮对非酒精性脂肪肝大鼠胰岛素抵抗的影响

Effect of Xiaoyuhuatan decoction on insulinresistance of nonalchoholic fatty liver disease rat

目的观察中药消瘀化痰饮对非酒精性脂肪肝（NAFLD）大鼠胰岛素抵抗的影响，探讨其防治NAFLD的作用机制。方法采用喂饲高脂饲料的方法复制大鼠胰岛素抵抗模型，以东宝肝泰为对照药，检测各组大鼠血清总胆固醇（TC）、甘油三酯（TG）、游离脂肪酸（FFA）、血清胰岛素（INS）、血糖（FBG）和肝组织中TC、TG的含量，计算胰岛素抵抗（IR）、胰岛素敏感指数（ISI）并观察肝组织形态学的变化。结果消瘀化痰饮能明显降低IR，增强ISI敏感性，降低模型大鼠血清TC、TG、FFA、FBG、INS及肝脏组织中TC、TG的含量（P〈0.05或P〈0．01），改善肝组织的病变程度。结论改善胰岛素抵抗、增强胰岛素敏感性可能是消瘀化痰饮防治NAFLD的作用机制之一。

Objective By observing the effect of Xiaoyuhuatan decoction on insulin resistance in rat with nonalchoholic fatty liver,the author made a reasearch into this herb＇s machanism on cambating on nonalchoholic fatty liver. Methods The insulin resistance rat model was fed by high fat forage and Dongbaogantai control group was set up for the sake of comparion. We determined the contents of total cholesterol（TC）, triglyceride（TG）, free fatty acid（FFA）, fasting plasma glucose（FBG）,fasting serum insulin（INS） in serum and the contents of TC, TG of liver tissue homogenate of each group,and the degree of hepatocytic steatosis. Results Xiaoyuhuatan decoction could obviously decrease the index of insulin resistance and improve insulin insensitive index. Lipid in blood serum and hepatic tissues were significantly decreased,liver fat cell denaturation was improved. Conclusion The thrapentic effect of Xiaoyuhuatan Decoction on fatty liver is probably contribute to this herb＇s mechanism of improving insulin resistance and increasing insulin insensitive.