白藜芦醇抑制晶体上皮细胞生长与诱导细胞凋亡的实验研究

Research on resveratrol＇s effect in suppressing growth and inducing apoptosis of lens epithelial cells

目的探讨白藜芦醇（Res）对晶体上皮细胞体外增殖的影响，观察Res诱导晶体上皮细胞凋亡的作用。方法用不同浓度的Res处理晶体上皮细胞，用四甲基偶氮唑蓝（MTT）比色法检测白藜芦醇对晶体上皮细胞增殖的影响，通过HE染色、电子显微镜、原位末端标记法（TUNEL）和流式细胞仪Annexin V荧光染色，观察细胞的形态学改变，并定量检测细胞凋亡。结果Res抑制了晶体上皮细胞的生长与增殖（P〈0．01），呈浓度依赖性反应；Res能明显诱导晶体上皮细胞凋亡，对照组与100μmol／L、200μmol／LRes处理24h后的细胞凋亡率分别为4．67％、17．31％、32．77％。结论Res能通过诱导晶体上皮细胞凋亡而抑制其生长与增殖，该研究为进一步探讨Res在白内障术后后囊膜混浊发生的防治提供了一定的实验依据。

Objective To explore the contribution of resveratrol（Res） to the proliferation and apoptosis of lens epithelial cells in vitro. Methods Methyl thiazolyl tetrazolium（MTT） assay was used to measure its effects on the proliferation of lens epithelial cells cultured with different concentrations of Res for 24h,48h and 72 h. HE staining,transmission electron microscope（TEM）,TUNEL fluorescence staining and the Annexin V assay by flow cytometer（FCM） were applied to observe the ceumorphological change detect the apoptosis induced by Res quantitatively. Results Res obviously suppressed the proliferation（P〈0.01） and induced the apoptosis of lens epithelial cells in concentration-dependent manner. The rate of apoptosis in control was 4.67 %, and 17.31%, 32.77 % after having been treated with 100μmol/L and 200μmol/L Res for 24h, respectively. Conclusion The results of this study confirm the ability of Res to suppress the growth and the proliferation of lens epithelial cells with a typical apoptotic feature in vitro. Therefore,Res might be considered as a possible treatment strategy for after-cataract.