摘要
建立HPLC法测定复方环丙沙星滴耳液中盐酸环丙沙星和氢化可的松的含量。采用LunaC,8色谱柱,流动相为甲醇-乙腈-0.34%磷酸二氢钾(25:15:60),流速1.0mL·min^-1,检测波长240nm。盐酸环丙沙星(按环丙沙星计)和氢化可的松的线性范围分别为0.1110~0.555mg·mL^-1,0.0108~0.0540mg·mL^-1;平均回收率分别为100.6%(RSD=0.2%)、99.8%(RSD=1.1%)。本法简便、快速、准确,专属性强,分离效果好。
A HPLC method was established for the determination of Ciprofloxacin and Hydrocortisone in Compound Ciprofloxacin Ear Drops. A Luna C18 Column was used with methol-Acetonitrile-0. 34% Potassium Dihydrogen phosphate(25 : 15 : 60) as mobile phase. The flow rate was 1.0mL·min^-1 and the detection wavelength was 240nm. The linear range were 0. 1110-0. 555mg· mL^-1 and 0. 0108-0. 0540 mg· mL^-1 for Ciprofloxacin and Hydrocortisone. The average recovery rete were 100.6% (RSD=0.2%) and 99.8%(RSD= 1.1%). The method is fast, simple, accurate, specific and with a good separating result.
出处
《中国药事》
CAS
2008年第3期242-243,共2页
Chinese Pharmaceutical Affairs