HPLC法测定止痢宁片中苦参碱与氧化苦参碱的含量

Determinnation of Matrine and Oxymatrine in Zhilining Tabets by HPLC

采用高效液相法同时测定止痢宁片中苦参碱和氧化苦参碱的含量。以Allttima Amino 100A为色谱柱，流动相：乙腈-无水乙醇-3％磷酸（78：11：11），流速：0．8mL·min^-1，检测波长：220nm。苦参碱的线性范围0．493-1．479μg，r=0．9997；氧化苦参碱的线性范围0．2054-0．7512μg，r=0．9994。平均加样回收率苦参碱为96．53％，RSD=1．83％；氧化苦参碱为95．02％，RSD=1．96％。本方法简便，专属，精密度好，分离效果好。

A HPLC method was established for the determinnation of matrine and oxymatrine in Zhilining Tablets. A Alhtima Amino 100A column was used with acetonitrile-ethyl alcohol-3% phosphoric acid solution （78 ： 11 ： 11） as mobile phase. The flow rate was 0. 8mL ·min^-1, and the detecting wavelength was 220nm. The liner range of matrine and Oxymatrine were 0. 4934 1. 479μg （r=0. 9997） and 0. 205440. 7512μg （r=0. 9994） respectively. The everage recovery rate and RSD were 95.63 %, 1.83 % and 95.02%, 1.96% respectively. The method is simple, specific, with good precision and separation result.