二次通用旋转试验设计优化黑曲霉固态发酵纤维素酶条件的研究

Studies on Optimizing the Conditions of Cellulase Fermentation by Aspergillus niger in Solid State through Quadratic General Rotary Design

采用二次通用旋转试验设计,对黑曲霉(Aspergillus niger sp.)固态发酵啤酒糟生产纤维素酶的条件进行了优化,并建立了纤维素酶随啤酒糟与棉粕的配料比、料水比和发酵时间变化的二次回归方程。利用该方程探讨了各因子对纤维素酶的影响。结果表明,各因子对纤维素酶的影响顺序为:配料比>发酵时间>料水比,而因子的交互作用不显著。利用统计优选法寻优,确定了最优发酵条件:啤酒糟与棉粕的配料比7:3,料水比1:1.5,发酵时间66 h,滤纸酶活最高值为782.4 u/g。

Cellulase production by Aspergillus niger sp.in solid state fermentation using brewers grain was optimized by quadratic general rotary design,and a quadratic regression equation was established.The effects of the factors were studied by the equation.The results showed the order of significance that affected the cellulase fermentation as the ratio of brewers grain to cotton cake,fermentation time,and the ratio of material to water.The mutual interaction of these factors was not significant.The statistical optimization method was used to optimize the fermentation conditions.The optimum conditions were：the ratio of brewers grain to cotton cake was 7：3,the ratio of material to water was 1：1.5,the time of fermentation was 66h, the maximal filter paper activity of the enzyme could reached 782.4μ/g （dry matter）.